2-[[(6-methyl-5-oxo-2H-1-2-4-triazin-3-yl)thio]methyl]-5-6-7-8-tetrahydro-3H-[1]benzothiolo[2-3-d]pyrimidin-4-one has been researched along with Inflammation* in 1 studies
1 other study(ies) available for 2-[[(6-methyl-5-oxo-2H-1-2-4-triazin-3-yl)thio]methyl]-5-6-7-8-tetrahydro-3H-[1]benzothiolo[2-3-d]pyrimidin-4-one and Inflammation
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Microsphere-based flow cytometry protease assays for use in protease activity detection and high-throughput screening.
This protocol describes microsphere-based protease assays for use in flow cytometry and high-throughput screening. This platform measures a loss of fluorescence from the surface of a microsphere due to the cleavage of an attached fluorescent protease substrate by a suitable protease enzyme. The assay format can be adapted to any site or protein-specific protease of interest and results can be measured in both real time and as endpoint fluorescence assays on a flow cytometer. Endpoint assays are easily adapted to microplate format for flow cytometry high-throughput analysis and inhibitor screening. Topics: Animals; Biotinylation; Flow Cytometry; Fluorescence Resonance Energy Transfer; Green Fluorescent Proteins; High-Throughput Screening Assays; Humans; Inflammation; Kinetics; Microspheres; Peptide Hydrolases; Peptides; Reproducibility of Results; Temperature | 2010 |